| Congresso Brasileiro de Microbiologia 2023 | Resumo: 804-1 | ||||
Resumo:The human gastrointestinal microbiome consists of billions of mostly beneficial microbes that are closely connected to human health. The stomach was long believed to be sterile due to its acidic environment but has recently been shown to harbor a distinct mucosal microbiota, often dominated by Helicobacter pylori. Several pathological conditions are connected to H. pylori infection, such as peptic ulcer disease and gastric cancer. Approximately 75% of cases of gastric cancers can be directly attributed to H. pylori, which is estimated to be carried by half of the world’s population. Interestingly, only a fraction of individuals will develop connected diseases; about 10% of individuals infected with H. pylori develop peptic ulcer disease, and only around 1-3% develop gastric cancer. Why H. pylori only cause disease in certain individuals is not fully understood.
In this study, we wanted to determine how interbacterial interactions might shape the colonization success of H. pylori in the stomach. In order to understand how H. pylori interact with other gastric bacteria, we have established a synthetic bacterial community representing the gastric microbiota. The synthetic community consists of five species: H. pylori CCUG 17875, Streptococcus salivarius CCUG 50207T, Pseudomonas aeruginosa PA01, Escherichia coli MG1655, and Lactobacillus kalixensis CCUG 48459T. These species were chosen based on either shown transcriptional activity in gastric biopsies or prevalence in gastric samples together with H. pylori.
All in all, this microbial model community will allow dissection of the genetic mechanisms behind H. pylori interactions with other bacteria in the gastric environment, how these interactions predispose certain individuals to disease, and how they influence H. pylori pathogenesis Palavras-chave: Gastric cancer, Microbial model communities, Peptic ulcers, SynComs | |||||